Analyzed the data: KSS XH.
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.Bacterial antimicrobial resistance is a critical public health issue of increasing importance for those who recreate and work in coastal regions. PLOS is a nonprofit 501(c)(3) corporation, #C2354500, based in San Francisco, California, US Abstract. Serotypes, biotypes, antibiotic susceptibilities were determined for 92 Vibrio cholerae isolates collected in 2011, 2012 and 2014 from Southern Ghana. Vibrio cholerae Scientific classification Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Vibrionales Family: Vibrionaceae Genus: Vibrio Species: V. cholerae Binomial name Vibrio cholerae Filippo Pacini & Robert Koch, 1854 Vibrio cholerae is a Gram-negative, comma-shaped bacterium.
Please enable it to take advantage of the complete set of features! 2014 Mar 21;14:269. doi: 10.1186/1471-2458-14-269. The bacterium's natural habitat is brackish or saltwater where they attach themselves easily to the chitin-containing shells of crabs, shrimps, and other shellfish. This site needs JavaScript to work properly.
This is counter to previous observations where percent antimicrobial resistance was elevated at sites contaminated with higher levels of enterococci that may have originated from fecal waste of humans This study represents the first investigation of antimicrobial susceptibility of We would like to thank Lara Kleinfelter and Bryan Shaw for laboratory assistance, Brian Sturgis and Eric Sherry (National Park Service at Assateague Island) and Dr. Eric May (University of Maryland Eastern Shore) for assistance in water collection and boat operation, Sabeena Nazar for assistance in sequencing isolates (BioAnalytical Services Laboratory, Institute of Marine and Environmental Technology), Matt Rhodes for technical assistance (Cooperative Oxford Laboratory), Dr. Craig Baker-Austin (Centre for Environment, Fisheries and Aquaculture Science, United Kingdom) for useful conversations regarding antimicrobial susceptibility testing, and Maryland Department of Health and Mental Hygiene scientists (Alvina Chu, Emily Ricotta, Dr. Robert Myers, Dr. Jafar Razeq, Celere Leonard) for sharing clinical isolates and associated metadata.Conceived and designed the experiments: KSS JMJ BCC ARS. Samples collected for Water-column depth and surface-water salinity, temperature, dissolved oxygen, conductivity, and pH were measured on every sampling date and at each location with a YSI 556 Multi-probe system (YSI Incorporated, Yellow Springs, OH) in accordance with the manufacturer's instructions.Fecal indicator measurements were conducted following the standard methods as described for enterococci in Standard Methods for the Examination of Water and Wastewater Surface water samples (100 µL) were spread plated in triplicate onto Chromagar Real-time PCR was performed by using 1X PCR Buffer (Qiagen, Valencia, CA), (Qiagen), 0.2 mM dNTP's solution (Qiagen), 1X Q solution (Qiagen), 2.25U TopTaq DNA polymerase (Qiagen), 75 nM internal control primers (each), 150 nM internal control probe, 2 µL internal control DNA, target primer and probe concentrations as detailed in A randomly chosen subset of isolates were taxonomically identified with 16 S rRNA gene sequences.
2019 Sep 11;19(1):801. doi: 10.1186/s12879-019-4432-2.Front Med (Lausanne). Most isolates were susceptible to antibiotics recommended for treating Vibrio infections, although the majority of isolates expressed intermediate resistance to chloramphenicol (78% of V. vulnificus, 96% of V. parahaemolyticus). Till now no studies have been done in Bidar to monitor the antibiotic susceptibility pattern of V. cholerae … Etiological agent of cholera is Vibrio cholerae which removes essential body fluids, salts and vital nutrients, which are necessary for life causing dehydration and malnutrition. Methodology/Principal Findings. Two sites, Sandy Point State Park and St. Martin's River, were characterized by frequent recreational use; and one site, the Pocomoke Sound, was characterized by heavy commercial fishing use ((Tracey Saxby, Kate Boicourt, Integration and Application Network, University of Maryland Center for Environmental Science (ian.umces.edu/imagelibrary/displayimage-127-5815.html).Sampling dates were chosen to coincide with times of high recreational and/or commercial use. Wrote the paper: KSS ARS BCC.For more information about PLOS Subject Areas, click Sandy Point State Park has historically been a site of low bacteriological water quality and is adjacent to the Magothy River, a site where there have been numerous wastewater treatment overflows.
Contributed reagents/materials/analysis tools: KSS RERG JMJ BCC ARS. Clipboard, Search History, and several other advanced features are temporarily unavailable.
Some strains of … eCollection 2019.Thapa Shrestha U, Adhikari N, Maharjan R, Banjara MR, Rijal KR, Basnyat SR, Agrawal VP.BMC Infect Dis.